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Orinoquia, Volumen 19, Número 2, p. 186-194, 2015. ISSN electrónico 2011-2629. ISSN impreso 0121-3709.

Nuevos genes relacionados con el sistema secretor tipo 4 de Edwardsiella tarda

Novel type 4 secretion system (T4SS)-related genes of Edwardsiella tarda

Noel Verjan-García, A. Carlos, Iregui Ikuo Hirono

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Resumen (es)

Edwardsiella tarda es una bacteria Gram-negativa responsable de edwardsiellosis, una enfermedad de peces y mamíferos incluido el humano, la cual se caracteriza por la formación de múltiples abscesos. Distintas cepas de E. tarda poseen un número de genes asociados con virulencia, resistencia a múltiples antibióticos y sistemas secretores de toxinas que explican en cierto grado su capacidad de sobrevivir dentro de células fagocíticas y de infectar a diversos hospederos. En estudios previos, nuestro grupo secuencio parcialmente el genoma de una cepa virulenta de E. tarda (ETSJ54) aislada de lenguado japonés (Paralichthys olivaceus) con edwardsiellosis y reportó un número de genes asociados a su virulencia. En este estudio se ha re-analizado los datos de secuenciación y en este proceso se identificaron varios genes que codifican para la estructura de superficie Pili y el sistema secretor tipo IV, la mayoría de los cuales estuvieron rodeados por genes codificadores de transposasas y otros genes de origen plasmídico. La secuencia de nucleótidos de dichos genes no tuvieron identidad con la de los genes previamente reportados en E. tarda EIB202, una cepa virulenta aislada de turbot (Scophthalmus maximus) en China. Los resultados sugieren diferencias en el contenido genético de cepas de E. tarda de distinto origen geográfico y la necesidad de desarrollar nuevos proyectos de secuenciamiento de genomas de E. tarda.

Palabras clave (es)

Pathogenesis; T4SS genes; Virulence; Patogenesis; Sistema secretor tipo IV; Virulencia

Resumen (en)

Edwardsiella tarda is a Gram-negative bacterium that causes edwardsiellosis, a disease of fish and mammals including humans and characterized by multiple abscesses. Different strains of E. tarda possess a number of virulence, antibioticresistance, and toxin secretion system-related genes that explain in some extent its capacity to survive within phagocytic cells and to infect a variety of hosts. Previously we introduced a virulent E. tardastrain (ETSJ54) isolated from Japanese flounder (Paralichthys olivaceus) with edwardsiellosis and reported a number of virulence-related genes. In this study we have re-analyzed the sequencing data of ETSJ54 and identified novel type IV secretion system-related genes, most of them were flanked by transposase and plasmid encoding genes. Interestingly, their nucleotide sequence had no identity to those of the genes published in the E. tarda EIB202 genome, a virulent strain isolated from turbot (Scophthalmus maximus) in China. The results suggest differences in gene content between geographically distinct E. tarda strains that may encourage additional E. tarda genome sequencing projects.

KeywordsPathogenesis; T4SS genes; Virulence.

Palabras clave (en)

Pathogenesis; T4SS genes; Virulence


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