Número actual Logotipo de Atom
Logotipo de RSS2
Logotipo de RSS1

Orinoquia, Volumen 8, Número 1, p. 25, 2004. ISSN electrónico 2011-2629. ISSN impreso 0121-3709.

Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed

DP Barajas, JG Velásquez, Poto A and Coy P

Texto completo:

Resumen (es)

ASBTRACT: The Murciano-levantina  cattle from the Spanish South-East belongs to a  bovine endangered breed under the special protection of FAO (WWL-DAD:3)*. Their characteristics of rusticity,  longe- vity, docility and disease resistance make interesting the recovery of the breed. The objective of the present work   was   to   deter mine   the efficiency of in vitro bovine embryo production using oocytes collected from post mortem ovaries fertilized with frozen thaw sperm obtained either from commercial or Murciano-levantina bulls. Cumulus oocyte complexes were matured for 24 h in TCM199 with 10% FBS, 2mM L-glutamine, 0.2 mM sodium pyruvate, 0.3  µg/mL LH and µg/mL FSH. Oocytes presumed matured  were fertilized with   Percoll-prepared   sper m (750.000/mL) in IVF-TALP as described by Parrish  et al. (Biol. Reprod  38:1171-1180, 1988). Zygotes (at 18 h) or two-cell stage embryos (at  48  h) were assessed by staining  with Hoechst 33342 (µg/mL; 20 min) under an epifluorescence microscopy. The experiment  was replicated  on 4 occasions.

The results  of penetration  (PEN-%), average number of spermato- zoa inside the oocyte (S/O), monos permy (MON- %) putative embryos  (zygotes with two pronuclei, 2 PN-%) and percentages of cleavage at 48 hours (Table 1) were recorded. The results show that early embryos can be obtained with no difference with the source of sperm used in the monospermy and the cleavage rate between commercial and Mur- ciano-levantina  sperm (p>0.05).

*Lista Mundial de Vigilancia para la Diversidad de los Animales Do- mésticos (1997). http:// www.fao.org/docrep/V8300S/ V8300S00.htm

Métricas de artículo

Vistas de resumen.
a description of the source 49

Cargando métricas ...

Enlaces refback

  • No hay ningún enlace refback.

2018 ® Universidad de los Llanos Nit: 892.000.757-3

Barcelona: Km. 12 Via Puerto López - PBX 6616800

San Antonio: Km. 12 Via Puerto López - PBX 6616900

Emporio: Km. 12 Via Puerto López - PBX 6616700

Fax: 6616800 ext 204

Horario de atención: Lunes a viernes 7:30am a 11:45am y 2:00pm a 5:30pm

Linea gratuita PQRs: 018000918641

Atención en línea: Lunes a viernes 7:30am a 11:45am y 2:00pm a 5:30pm



Fax: 6616800 ext 204

Políticas de privacidad y términos de uso