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Orinoquia, Volumen 24, Número 2, p. 51-78, 2020. ISSN electrónico 2011-2629. ISSN impreso 0121-3709.

Crioconservación seminal en peces de agua dulce: aspectos biotecnológicos, celulares y bioquímicos

Seminal Cryopreservation in Freshwater Fish: Biotechnological, Cellular and Biochemical Aspects

Criopreservação Seminal em Peixes de Água Doce: Aspectos Biotecnológicos, Celulares e Bioquímicos

Víctor M. Medina-Robles, Astrid S. Duarte-Trujillo, Pablo E. Cruz-Casallas

Texto completo:


Resumen (es)

La crioconservación es una herramienta biotecnológica que en peces está orientada principalmente a la conservación criogénica de semen como estrategia de preservación del recurso genético y a su uso para la producción de alevinos con fines diferentes. Actualmente, los protocolos de crioconservación seminal en peces de agua dulce establecen una amplia variedad de procedimientos cuya efectividad se basa en aspectos ligados a la calidad seminal post-descongelación y la fertilidad, así como su relación con el desarrollo de la progenie. El efecto de la conservación del semen en nitrógeno líquido por periodos amplios de tiempo también toma importancia en ésta biotecnología. Por lo anterior, el objetivo de la presente revisión es describir aspectos biotecnológicos, celulares y bioquímicos asociados al proceso de crioconservación seminal en peces dulceacuícolas, resaltando los avances, las limitaciones y sus perspectivas.

Palabras clave (es)

criobiología; crioconservación; espermatozoide; peces; semen

Resumen (en)

Cryopreservation is a biotechnological tool that in fish is mainly aimed at cryogenic conservation of semen as a strategy for preserving the genetic resource and its use for the production of fingerlings with different purposes. Currently, seminal cryopreservation protocols in freshwater fish establish a wide variety of procedures whose effectiveness is based on aspects linked to seminal post-thaw quality and fertility, as well as its relationship with the development of the progeny. The effect of preserving semen in liquid nitrogen for extended periods of time also plays an important role in this biotechnology. Therefore, the objective of this review is to describe biotechnological, cellular and biochemical aspects associated with the seminal cryopreservation process in freshwater fish, highlighting the advances, limitations and perspectives.

Palabras clave (en)

cryobiology; cryopreservation; spermatozoa; fish; semen

Resumen (pt)

A criopreservação é uma ferramenta biotecnológica que em peixes visa principalmente a conservação criogênica do sêmen como estratégia para a preservação do recurso genético e sua utilização para a produção de alevinos para diferentes fins. Atualmente, os protocolos de criopreservação seminal em peixes de água doce estabelecem uma ampla variedade de procedimentos cuja eficácia se baseia em aspectos relacionados à qualidade e fertilidade pós-descongelamento seminal, bem como sua relação com o desenvolvimento da progênie. O efeito da preservação do sêmen no nitrogênio líquido por longos períodos de tempo também desempenha um papel importante nessa biotecnologia. Portanto, o objetivo desta revisão é descrever aspectos biotecnológicos, celulares e bioquímicos associados ao processo de criopreservação seminal em peixes de água doce, destacando os avanços, limitações e perspectivas.

Palabras clave (pt)

criobiologia; criopreservação; esperma; peixe; sêmen

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