Antioxidantes en la crioconservación de semen de peces: una revisión con énfasis en especies de agua dulce de Sur América

Antioxidants in the cryopreservation of fish semen: a review with emphasis on freshwater species from south America

Contenido principal del artículo

Resumen

El rápido crecimiento de la población mundial ha conducido a una sobreexplotación de los recursos naturales y, los recursos hídricos no son la excepción; afectando las poblaciones de peces en todo el mundo. Además, la poca variabilidad en las especies de interés comercial y los pocos avances en el desarrollo de paquetes tecnológicos y productivos conllevan a que esta problemática se acentúe. La crioconservación seminal es una técnica que permite el resguardo del material genético durante tiempo indefinido, permitiendo su disponibilidad constante. Sin embargo, puede causar algunos efectos negativos sobre la integridad celular y sus funciones. Dentro de esto, la formación de cristales de hielo, el estrés osmótico y con gran relevancia el estrés oxidativo son los de mayor incidencia. De acuerdo a lo anterior, el uso de sustancias con capacidad de reducir los efectos del estrés oxidativo como lo son los antioxidantes pueden constituirse como una alternativa de mejora de estos procesos conllevando a la estandarización de protocolos mejorados para su aplicación en bancos de germoplasma. El objetivo de esta revisión es hacer una breve descripción de la crioconservación seminal como biotecnología reproductiva, sus usos e implicaciones y de algunos de los trabajos desarrollados en especies nativas de Sur América con el uso de sustancias antioxidantes.

Descargas

Los datos de descargas todavía no están disponibles.

Detalles del artículo

Biografía del autor/a (VER)

Diana Guaje, University of the Llanos

Estudiante de Maestría en Acuicultura, Grupo de Investigación sobre Reproducción y Toxicología de Organismos Acuáticos – GRITOX, Instituto de Acuicultura – IALL, Facultad de Ciencias Agropecuarias y Recursos Naturales, Universidad de los Llanos, Villavicencio, Colombia, Email: [email protected]

Víctor M. Medina-Robles, University of the Llanos

Grupo de Investigación sobre Reproducción y Toxicología de Organismos

Acuáticos – GRITOX, Instituto de Acuicultura – IALL, Facultad de Ciencias

Agropecuarias y Recursos Naturales, Universidad de los Llanos, Villavicencio, Colombia, Email: [email protected]

Referencias (VER)

Adames, M. S., de Toledo, C. P. R., Neumann, G., Buzzi, A. H., Buratto, C. N., Piana, P. A., y Bombardelli, R. A. (2015). Optimization of the sperm: Oocyte ratio and sperm economy in the artificial reproduction of Rhamdia quelen using fructose as a sperm motility modulator. Animal Reproduction Science, 161, 119–128. https://doi.org/10.1016/j.anireprosci.2015.08.014

Agarwal, A. y Prabakaran, S. A. (2005). Oxidative stress and antioxidants in male infertility: a difficult balance. Iranian Journal of Reproductive Medicine Iranian Journal of Reproductive Medicine, 3(1), 1–8. http://ssu.ac.ir/ijrm/index.php/ijrm/article/view/392

Aisen, E., Quintana, M., Medina, V., Morello, H., y Venturino, A. (2005). Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose-based hypertonic extenders. Cryobiology, 50(3), 239–249. https://doi.org/10.1016/j.cryobiol.2005.02.002

Alves Pereira, F. (2015). Glutationa Reduzida e Adenosina trifosfato (ATP) na Criopreservação seminal de Tambaqui, Colossoma macropomum. Tese de Mestre. Brasil, Universidade Federal do Rio Grande.

Ball, B. A. (2008). Oxidative stress, osmotic stress and apoptosis: Impacts on sperm function and preservation in the horse. Animal Reproduction Science, 107(3–4), 257–267. https://doi.org/10.1016/j.anireprosci.2008.04.014

Bansal, A. K., y Bilaspuri, G. S. (2011). Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International, 2011. https://doi.org/10.4061/2011/686137

Cabrita, E., Horváth, Á., Marinović, Z., & Asturiano, J. F. (2022). Technologies and strategies for ex situ conservation of aquatic organisms: the role of cryopreservation in long-term management. In Cellular and Molecular Approaches in Fish Biology (pp. 1-48). Academic Press. Barreiros, A. L. B. S.,

David, J. M., y David, J. P. (2006). Estresse oxidativo: relação entre geração de espécies reativas e defesa do organismo. Quimica Nova, 29(1), 113–123. https://doi.org/10.1590/s0100-40422006000100021

Betsy, J., y Kumar, S. (2020). Cryopreservation of Fish Gametes. https://doi.org/10.1007/978-981-15-4025-7_3

Bilodeau, J. F., Blanchette, S., Gagnon, C., y Sirard, M. A. (2001). Thiols prevent H2O2-mediated loss of sperm motility in cryopreserved bull semen. Theriogenology, 56(2), 275–286. https://doi.org/10.1016/S0093-691X(01)00562-3

Bucak, M. N., Ateşşahin, A., Varişli, Ö., Yüce, A., Tekin, N., y Akçay, A. (2007). The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen. Microscopic and oxidative stress parameters after freeze-thawing process.

Theriogenology, 67(5), 1060–1067. https://doi.org/10.1016/j.theriogenology.2006.12.004

Bunaciu, A. A., Aboul-Enein, H. Y., y Fleschin, S. (2012). FTIR spectrophotometric methods used for antioxidant activity assay in medicinal plants. Applied Spectroscopy Reviews, 47(4), 245–255. https://doi.org/10.1080/05704928.2011.645260

Cabrita, E., Martínez-Páramo, S., Gavaia, P. J., Riesco, M. F., Valcarce, D. G., Sarasquete, C., Herráez, M. P., y Robles, V. (2014). Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture, 432, 389–401. https://doi.org/10.1016/j.aquaculture.2014.04.034

Cabrita, E., Robles, V., Cuñado, S., Wallace, J. C., Sarasquete, C., y Herráez, M. P. (2005). Evaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5 ml macrotubes. Cryobiology, 50(3), 273–284. https://doi.org/10.1016/j.cryobiol.2005.02.005

Cabrita, E., Sarasquete, C., Martínez-Páramo, S., Robles, V., Beirão, J., Pérez-Cerezales, S., y Herráez, M. P. (2010). Cryopreservation of fish sperm: Applications and perspectives. Journal of Applied Ichthyology, 26(5), 623–635. https://doi.org/10.1111/j.1439-0426.2010.01556.x

Calcagnotto, D., y De Almeida Toledo-Filho, S. (2000). Loss of genetic variability at the transferrin locus in five hatchery stocks of tambaqui (Colossoma macropomum). Genetics and Molecular Biology, 23(1), 127–130. https://doi.org/10.1590/S1415-47572000000100023

Cao, G., y Cutler, R. G. (1993). High concentrations of antioxidants may not improve defense against oxidative stress. Archives of Gerontology and Geriatrics, 17(3), 189–201. https://doi.org/10.1016/0167-4943(93)90050-R

Carvalho, O. F. de, Ferreira, J. D. de J., Silveira, N. de A., y Freneau, G. E. (2002). Efeito oxidativo do óxido nítrico e infertilidade no macho. Jornal Brasileiro de Patologia e Medicina Laboratorial, 38(1), 33–38. https://doi.org/10.1590/s1676-24442002000100007

Chapman, B. B., Hulthén, K., Brodersen, J., Nilsson, P. A., Skov, C., Hansson, L. A., y Brönmark, C. (2012). Partial migration in fishes: Causes and consequences. Journal of Fish Biology, 81(2), 456–478. https://doi.org/10.1111/j.1095-8649.2012.03342.x

Cosson, J. (2019). Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation. In Biological Research in Aquatic Science. https://doi.org/10.5772/intechopen.85139

Costa LS, Fidelis GP, Cordeiro SL, et al.(2010) Biological activities of sulfated polysaccharides from tropical seaweeds. Biomed Pharmacother, 64(1), 21–28. doi:10.1016/j.biopha.2009.03.005

Cruzat, V. F., Petry, É. R., y Tirapegui, J. (2009). Glutamina: aspectos bioquímicos, metabólicos, moleculares e suplementação. Revista Brasileira de Medicina Do Esporte, 15(5), 392–397. https://doi.org/10.1590/s1517-86922009000600015

Da Costa, B. B., Marques, L. S., Lassen, P. G., Rodrigues, R. B., Da Rosa-Silva, H. T., Moreira, J. C. F., de Oliveira, D. L., y Streit, D. P. (2020). Effect of glutamine and cysteine supplementation on quality of cryopreserved sperm of South American silver catfish. Aquaculture Research, 52(5), 2173–2181. https://doi.org/10.1111/are.15070

Da Costa, B. B., Marques, L. S., Lassen, P. G., Rodrigues, R. B., Tais Da Rosa Silva, H., Moreira, J. C. F., y Streit, D. P. (2019). Effects of cysteine supplementation on the quality of cryopreserved sperm of South American silver catfish. Aquaculture Research, 51(2), 455–464. https://doi.org/10.1111/are.14389

Da Silva, E. C. B., y Guerra, M. M. P. (2012). Terapias antioxidantes na criopreservação espermática. Revista Portuguesa de Ciências Veterinárias, 111, 143–149.

De Almeida-Monteiro, P. S., Oliveira-Araújo, M. S., Pinheiro, R. R. R., Lopes, J. T., Ferreira, Y. M., Montenegro, A. R., Melo-Maciel, M. A. P., y Salmito-Vanderley, C. S. B. (2017). Influence of vitamins C and e on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei). Semina: Ciencias Agrarias, 38(4), 2669–2680. https://doi.org/10.5433/1679-0359.2017v38n4Supl1p2669

de Oliveira Pedreira, A. C., Malacarne, A. M., Dalmaso, A. C. S., Carvalho, K. I. F. S., Chagas, T. V., da Silva Gambetta, M. I. R., Chiella, R. J., y Bombardelli, R. A. (2022). L-carnitine solution used on Rhamdia quelen thawed sperm activation boosts sperm movement, maintains larval quality, and permits to optimize the sperm use. Animal Reproduction Science, 245. https://doi.org/10.1016/j.anireprosci.2022.107054

Dickinson, D. A., y Forman, H. J. (2002). Cellular glutathione and thiols metabolism. Biochemical Pharmacology, 64, 1019–1026. https://doi.org/https://doi.org/10.1016/S0006-2952(02)01172-3

Dourado, O. F. (1981). Principais peixes e crustáceos dos açudes controlados pelo DNOCS. Convênio SUDENE/DNOCS.

Félix, F., Oliveira, C. C. V., y Cabrita, E. (2021). Antioxidants in fish sperm and the potential role of melatonin. Antioxidants, 10(1), 1–29. https://doi.org/10.3390/antiox10010036

Fidalgo-Guerreiro, V. H., y FERREIRA, G. (2011). Mitigação de impactos à ictiofauna após barramentos de corpos d’água através de medidas socioeducativas e educação ambiental. In 1 CONGRESSO BRASILEIRO DE AVALIAÇÃO DE IMPACTO (Vol. 1, p. 2011).

Figueroa, E., Farias, J. G., Lee-Estevez, M., Valdebenito, I., Risopatrón, J., Magnotti, C., Romero, J., Watanabe, I., y Oliveira, R. P. S. (2018). Sperm cryopreservation with supplementation of α-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar). Aquaculture, 493, 1–8. https://doi.org/10.1016/j.aquaculture.2018.04.046

Food and Agriculture Organization (FAO). (2022). El estado mundial de la pesca y la acuicultura 2022. Hacia la transformación azul. In Fao. https://doi.org/https://doi.org/10.4060/cc0461

Food and Agriculture Organization of the United Nations (FAO). (2018). The State of World Fisheries and Aquaculture. Retrieved from http://www.fao.org/3/i9540en/i9540en.pdf

Galo, J. M., Streit-Junior, D. P., Sirol, R. N., Ribeiro, R. P., Digmayer, M., Andrade, V. X. L., y Ebert, A. R. (2011). Anormalidades espermáticas de piracanjuba Brycon orbignyanus (Valenciennes, 1849) após a criopreservação. Brazilian Journal of Biology, 71(3), 693–699. https://doi.org/10.1590/S1519-69842011000400014

Gheller, S. M. M., Corcini, C. D., de Brito, C. R. C., Acosta, I. B., Tavares, G. C., Soares, S. L., Silva, A. C., Pires, D. M., y Varela Junior, A. S. (2019). Use of trehalose in the semen cryopreservation of Amazonian catfish Leiarius marmoratus. Cryobiology, 87(June 2018), 74–77. https://doi.org/10.1016/j.cryobiol.2019.02.001

Gülçin, I. (2006). Antioxidant and antiradical activities of L-carnitine. Life Sciences, 78(8), 803–811. https://doi.org/10.1016/j.lfs.2005.05.103

Hernández, C. L., Ortega-Lara, A., Sánchez-Garcés, G. C., y Alford, M. H. (2015). Genetic and Morphometric Evidence for the Recognition of Several Recently Synonymized Species of Trans-Andean Rhamdia (Pisces: Siluriformes: Heptapteridae). Copeia, 103(3), 563–579. https://doi.org/10.1643/CI-14-145

Holt, W. V. (2000a). Basic aspects of frozen storage of semen. Animal Reproduction Science, 62(1–3), 3–22. https://doi.org/10.1016/S0378-4320(00)00152-4

Holt, W. V. (2000b). Fundamental aspects of sperm cryobiology: The importance of species and individual differences. Theriogenology, 53(1), 47–58. https://doi.org/10.1016/S0093-691X(99)00239-3

Horizonte, B., Borges, J. C., Silva, M. R., Esper, C. R., y Franceschini, P. H. (2011). Membrana plasmática de espermatozoides bovinos: efeito de metabólitos do oxigênio, antioxidantes e criopreservação. Revista Brasileira de Reprodução Animal, 35(3), 303–314.

Klaiwattana, P., Srisook, K., Srisook, E., Vuthiphandchai, V., y Neumvonk, J. (2016). Effect of cryopreservation on lipid composition and antioxidant enzyme activity of seabass (Lates calcarifer) sperm. Iranian Journal of Fisheries Sciences, 15(1), 157–169. http://www.jifro.ir/browse.php?a_id=940&sid=1&slc_lang=en%0Ahttps://www.cabdirect.org/cabdirect/abstract/20163044938

Kohen, R., y Nyska, A. (2002). Oxidation of Biological Systems: Oxidative Stress Phenomena, Antioxidants, Redox Reactions, and Methods for Their Quantification. Toxicologic Pathology, 30(6), 620–650. https://doi.org/10.1080/0192623029016672

Lahnsteiner, F., y Caberlotto, S. (2012). Motility of gilthead seabream Sparus aurata spermatozoa and its relation to temperature, energy metabolism and oxidative stress. Aquaculture, 370–371, 76–83. https://doi.org/10.1016/j.aquaculture.2012.09.034

Lasso, L., Alvarez, G., y June, M. (1994). of Superoxide Cells during Cryopreservation. 15(3).

Lima Assis, I. D., Palhares, P. C., Machado, G. J., Souza, J. G. D. S., Souza França, T. D., Oliveira Felizardo, V. D., y Murgas, L. D. S. (2019). Effect of melatonin on cryopreserved sperm of Prochilodus lineatus (Characiformes). CryoLetters, 40(3), 152-158.

Lopes, J.T., Salmito-Vanderley, C.S.B., Almeida-Monteiro, P. S. (2016). Presença de antioxidantes no sêmen de teleósteos e sua utilização na suplementação de meios de congelação seminal. Revista Brasileira de Reprodução Animal, 40(1), 29–34.

Lopes, J. T., Oliveira-Araújo, M. S., Nascimento, R. V. do, Montenegro, Y. M. F. A. R., y Salmito-Vanderley, C. S. B. (2018). Efeito de vitaminas e aminoácidos como suplementação da solução crioprotetora para a criopreservação do sêmen de tambaqui (Colossoma macropomum). In Acta Scientiae Veterinariae (Vol. 46, Issue August, pp. 1–8).

Luberda, Z. (2005). The role of glutathione in mammalian gametes. Reproductive Biology, 5(1), 5–17.

Maldonado-Ocampo, J., Vari, R., y Usma Oviedo, J. S. (2008). Checklist of the freshwater fishes of Colombia. Biota Colombiana, 9(2), 312.

Marchioro, M. I., y Baldisserotto, B. (1999). Sobrevivência de alevinos de Jundiá (Rhamdia quelen Quoy & Gaimard, 1824) à variação de salinidade da água. Ciência Rural, 29(2), 315–318. https://doi.org/10.1590/s0103-84781999000200021

Maria, A. N., Azevedo, H. C., & Carneiro, P. C. F. (2011). Protocolo para criopreservação do sêmen de Tambaqui (Colossoma macropomum). Comunicado Técnico, 112, 8.

Martínez-Páramo, S., Diogo, P., Dinis, M. T., Herráez, M. P., Sarasquete, C., y Cabrita, E. (2012). Incorporation of ascorbic acid and α-tocopherol to the extender media to enhance antioxidant system of cryopreserved sea bass sperm. Theriogenology, 77(6), 1129–1136. https://doi.org/10.1016/j.theriogenology.2011.10.017

Mazur, P. (1984). Freezing of living cells: mechanisms and implications. The American Journal of Physiology, 247(3 Pt 1), 0–4. https://doi.org/10.1152/ajpcell.1984.247.3.C125

Medeiros, G. F., Mendes, A., Castro, R. A. B., Baú, E. C., Nader, H. B., y Dietrich, C. P. (2000). Distribution of sulfated glycosaminoglycans in the animal kingdom: Widespread occurrence of heparin-like compounds in invertebrates. Biochimica et Biophysica Acta - General Subjects, 1475(3), 287–294. https://doi.org/10.1016/S0304-4165(00)00079-9

Medina-Robles, V. M., Velasco-Santamaría, Y. M., & Cruz-Casallas, P. E. (2005). Aspectos generales de la crioconservación espermática en peces teleósteos. Revista Colombiana de Ciencias Pecuarias, 18(1), 34–48. file:///C:/Users/Cris/Desktop/REFER?NCIAS/Robles, Santamar?a, Casallas - Aspectos generales de la crioconservaci?n esperm?tica en peces tele?steos - 2005.pdf

Migaud, H., Bell, G., Cabrita, E., McAndrew, B., Davie, A., Bobe, J., ... & Carrillo, M. (2013). Gamete quality and broodstock management in temperate fish. Reviews in Aquaculture, 5, S194-S223.

Miliorini, A. B., Murgas, L. D. S., Rosa, P. V., Oberlender, G., Pereira, G. J. M., y Da Costa, D. V. (2011). A morphological classification proposal for curimba (Prochilodus lineatus) sperm damages after cryopreservation. Aquaculture Research, 42(2), 177–187. https://doi.org/10.1111/j.1365-2109.2010.02575.x

Mojica, J. I., Usma Oviedo, J. S., Álvarez León, R., y Lasso, C. A. (2012). Libro rojo de peces dulceacuícolas.

Motta, N. C., Egger, R. C., Monteiro, K. S., Vogel de Oliveira, A., y Solis Murgas, L. D. (2022). Effects of melatonin supplementation on the quality of cryopreserved sperm in the neotropical fish Prochilodus lineatus. Theriogenology, 179, 14–21. https://doi.org/10.1016/j.theriogenology.2021.11.012

Nascimento, R. V. do. (2021). Suplementação do meio de congelação seminal de Prochilodus brevis com polissacarídeos sulfatados extraídos de pele de tilápia e de algas marinhas. Tese de Doutorado. Brasil, Universidade estadual do ceará, 2021. https://pesquisa.bvsalud.org/portal/resource/pt/vtt-221996

Navarro, R. D., Navarro, F. K. S. P., Felizardo, V. de O., Murgas, L. D. S., y Andrade, E. de S. (2014). Qualidade de sêmen de Curimba (Prochilodus lineatus) criopreservados com vitaminas. Acta Scientiarum - Technology, 36(1), 55–60. https://doi.org/10.4025/actascitechnol.v36i1.19586

Nordberg, J., y Arnér, E. S. J. (2001). Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radical Biology and Medicine, 31(11), 1287–1312. https://doi.org/10.1016/S0891-5849(01)00724-9

Olfati Karaji, R., Daghigh Kia, H., y Ashrafi, I. (2014). Effects of in combination antioxidant supplementation on microscopic and oxidative parameters of freeze-thaw bull sperm. Cell and Tissue Banking, 15(3), 461–470. https://doi.org/10.1007/s10561-013-9412-y

Palhares, P. C., Assis, I. de L., Machado, G. J., de Freitas, R. M. P., de Freitas, M. B. D., Paula, D. A. J., Carneiro, W. F., Motta, N. C., y Murgas, L. D. S. (2021). Sperm characteristics, peroxidation lipid and antioxidant enzyme activity changes in milt of Brycon orbignyanus cryopreserved with melatonin in different freezing curves. Theriogenology, 176, 18–25. https://doi.org/10.1016/j.theriogenology.2021.09.013

Palhares, P. C., Assis, I. de L., Souza, J. G. da S., França, T. de S., Egger, R. C., Paula, D. A. de J., y Murgas, L. D. S. (2020). Effect of melatonin supplementation to a cytoprotective medium on post-thawed Brycon orbignyanus sperm quality preserved during different freezing times. Cryobiology, 96, 159–165. https://doi.org/10.1016/j.cryobiol.2020.07.002

Paula, D. A. J., Andrade, E. S., Murgas, L. D. S., Felizardo, V. O., Winkaler, E. U., Zeviani, W., y Freitas, R. T. F. (2012). Vitamin E and reduced glutathione in Prochilodus lineatus (curimba) semen cryopreservation (Characiformes: Prochilodontidae). Neotropical Ichthyology, 10(3), 661–665. https://doi.org/10.1590/S1679-62252012005000016

Pereira Maduenho, L., y Martinez, C. B. R. (2008). Acute effects of diflubenzuron on the freshwater fish Prochilodus lineatus. Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, 148(3), 265–272. https://doi.org/10.1016/j.cbpc.2008.06.010

Pereira, V. A., de Alencar, D. B., Araújo, I. W. F. de, Rodrigues, J. A. G., Lopes, J. T., Nunes, L. T., Ferreira, Y. M., Lobato, J. S., Montenegro, A. R., y Salmito Vanderley, C. S. B. (2020). Supplementation of cryodiluent media with seaweed or Nile tilapia skin sulfated polysaccharides for freezing of Colossoma macropomum (Characiformes: Serrasalmidae) semen. Aquaculture, 528. https://doi.org/10.1016/j.aquaculture.2020.735553

Reiter, R. J. (2000). Melatonin: Lowering the high price of free radicals. News in Physiological Sciences, 15(5), 246–250. https://doi.org/10.1152/physiologyonline.2000.15.5.246

Costa, L. S., Fidelis, G. P., Cordeiro, S. L., Oliveira, R. M., Sabry, D. A., Câmara, R. B. G., Nobre, L. T. D. B., Costa, M. S. S. P., Almeida-Lima, J., Farias, E. H. C., Leite, E. L., & Rocha, H. A. O. (2010). Biological activities of sulfated polysaccharides from tropical seaweeds. Biomedicine and Pharmacotherapy, 64(1), 21–28. https://doi.org/10.1016/j.biopha.2009.03.005

Gadea, J., Molla, M., Selles, E., Marco, M. A., Garcia-Vazquez, F. A., & Gardon, J. C. (2011). Reduced glutathione content in human sperm is decreased after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders. Cryobiology, 62(1), 40–46. https://doi.org/10.1016/j.cryobiol.2010.12.001

Medina-Robles, V. M., Velasco-Santamaría, Y. M., & Cruz-Casallas, P. E. (2005). Aspectos generales de la crioconservación espermática en peces teleósteos. Revista Colombiana de Ciencias Pecuarias, 18(1), 34–48. file:///C:/Users/Cris/Desktop/REFER?NCIAS/Robles, Santamar?a, Casallas - Aspectos generales de la crioconservaci?n esperm?tica en peces tele?steos - 2005.pdf

Reis, R. E., Albert, J. S., Di Dario, F., Mincarone, M. M., Petry, P., & Rocha, L. A. (2016). Fish biodiversity and conservation in South America. Journal of fish biology, 89(1), 12–47. https://doi.org/10.1111/jfb.13016

Reynalte-Tataje, D. A., Soares, M. da L., Massaro, M. V., Bastian, R., y Pelicice, F. M. (2020). First evidence of a spawning site of the endangered fish Brycon orbignyanus (Valenciennes, 1850) (characiformes, bryconidae) in the middlE Uruguay River, Brazil. Acta Limnologica Brasiliensia, 32(3 m), 1–5. https://doi.org/10.1590/S2179-975X2220

Ricardo, M. C., Aguiar, C. A., Rizzo, E., y Bazzoli, N. (1996). Morfologia da micrópila e da células micropilar em teleósteos neotropicais de água doce. Arq. Bras. Med. Vet. Zootec, 17-24.

Saleh, R. A., y Agarwal, A. (2002). Oxidative stress and male infertility: From research bench to clinical practice. Journal of Andrology, 23(6), 737–752.

Sanches, E. G., Tosta, G. A. M., y Souza-Filho, J. J. (2013). Economic feasibility of cobia juvenile production (Rachycentron canadum). Boletim Do Instituto de Pesca, 39(1), 15–26.

Sandoval-Vargas, L., Dumorné, K., Contreras, P., Farías, J. G., Figueroa, E., Risopatrón, J., y Valdebenito, I. (2021). Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity. Aquaculture, 533(September). https://doi.org/10.1016/j.aquaculture.2020.736151

Sanocka, D., y Kurpisz, M. (2004). Reactive oxygen species and sperm cells. Reproductive Biology and Endocrinology, 2(Table 2), 1–7. https://doi.org/10.1186/1477-7827-2-12

Sikka, S. (2001). Relative Impact of Oxidative Stress on Male Reproductive Function. Current Medicinal Chemistry, 8(7), 851–862. https://doi.org/10.2174/0929867013373039

Steiber, A., Kerner, J., y Hoppel, C. L. (2004). Carnitine: A nutritional, biosynthetic, and functional perspective. Molecular Aspects of Medicine, 25(5–6), 455–473. https://doi.org/10.1016/j.mam.2004.06.006

Streit, D. P., Sirol, R. N., Ribeiro, R. P., Moraes, G. V., Vargas, L. D. M., y Watanabe, A. L. (2008). Qualitative parameters of the piapara semen (Leporinus elongatus Valenciennes, 1850). Brazilian Journal of Biology, 68(2), 373–377. https://doi.org/10.1590/S1519-69842008000200019

Suquet, M., Dreanno, C., Fauvel, C., Cosson, J., y Billard, R. (2000). Cryopreservation of sperm in marine fish. Aquaculture Research, 31(3), 231–243. https://doi.org/10.1046/j.1365-2109.2000.00445.x

Xavier, A. M. M., Neumann, G., Sanches, E. A., Cardoso, S. U., y Bombardelli, R. A. (2021). Extenders with vitamins C and E applied to Rhamdia quelen sperm cryopreservation / Extensores com vitaminas C e aplicados à criopreservação de esperma de Rhamdia quelen. Brazilian Journal of Development, 7(12), 119898–119912. https://doi.org/10.34117/bjdv7n12-653

Zhu, Z., Li, R., Lv, Y., y Zeng, W. (2019). Melatonin protects rabbit spermatozoa from cryo-damage via decreasing oxidative stress. Cryobiology, 88(22), 1–8. https://doi.org/10.1016/j.cryobiol.2019.04.009

Zini, A., y Libman, J. (2014). Oxidative Stress and Male Infertility. Systems Biology of Free Radicals and Antioxidants, 9783642300, 1–4178. https://doi.org/10.1007/978-3-642-30018-9

Artículos más leídos del mismo autor/a

1 2 > >>